| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KANK2 (1-300) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KANK2 |
| Gene ID: |
25959 |
| Uniprot ID: |
KANK2_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human KANK2 (NP_001129663.1). |
| Immunogen Sequence: |
MAQVLHVPAPFPGTPGPASP PAFPAKDPDPPYSVETPYGY RLDLDFLKYVDDIEKGHTLR RVAVQRRPRLSSLPRGPGSW WTSTESLCSNASGDSRHSAY SYCGRGFYPQYGALETRGGF NPRVERTLLDARRRLEDQAA TPTGLGSLTPSAAGSTASLV GVGLPPPTPRSSGLSTPVPP SAGHLAHVREQMAGALRKLR QLEEQVKLIPVLQVKLSVLQ EEKRQLTVQLKSQKFLGHP |
| Tissue Specificity | Strongly expressed in cervix, colon, heart, kidney and lung. Expressed in kidney glomerular podocytes and mesangial cells (at protein level). |
| Post Translational Modifications | Phosphorylated by casein kinase II upon estrogen stimulation. Phosphorylation induces the release by KANK2 of NCOA1 and its translocation to the nucleus where NCOA1 can activate gene transcription. |
| Function | Involved in transcription regulation by sequestering in the cytoplasm nuclear receptor coactivators such as NCOA1, NCOA2 and NCOA3. Involved in regulation of caspase-independent apoptosis by sequestering the proapoptotic factor AIFM1 in mitochondria. Pro-apoptotic stimuli can induce its proteasomal degradation allowing the translocation of AIFM1 to the nucleus to induce apoptosis. Involved in the negative control of vitamin D receptor signaling pathway. Involved in actin stress fibers formation through its interaction with ARHGDIA and the regulation of the Rho signaling pathway. May thereby play a role in cell adhesion and migration, regulating for instance podocytes migration during development of the kidney. Through the Rho signaling pathway may also regulate cell proliferation. |
| Protein Name | Kn Motif And Ankyrin Repeat Domain-Containing Protein 2Ankyrin Repeat Domain-Containing Protein 25Matrix-Remodeling-Associated Protein 3Src-1-Interacting ProteinSipSrc-Interacting ProteinSrc1-Interacting Protein |
| Cellular Localisation | CytoplasmMitochondrion |
| Alternative Antibody Names | Anti-Kn Motif And Ankyrin Repeat Domain-Containing Protein 2 antibodyAnti-Ankyrin Repeat Domain-Containing Protein 25 antibodyAnti-Matrix-Remodeling-Associated Protein 3 antibodyAnti-Src-1-Interacting Protein antibodyAnti-Sip antibodyAnti-Src-Interacting Protein antibodyAnti-Src1-Interacting Protein antibodyAnti-KANK2 antibodyAnti-ANKRD25 antibodyAnti-KIAA1518 antibodyAnti-MXRA3 antibodyAnti-SIP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
