Anti-JPH2 antibody (611-660 aa) (STJ192568)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 611-660
General Information
| Short Description | Rabbit polyclonal anti-Junctophilin-2 (611-660 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | JPH2 |
| Gene ID | 57158 |
| Uniprot ID | JPH2_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 611-660 |
| Immunogen Region | 611-660 aa |
| Specificity | JPH2 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Phosphorylation on Ser-165, probably by PKC, affects RYR1-mediated calcium ion release, interaction with TRPC3, and skeletal muscle myotubule development. Proteolytically cleaved by calpain in response to cardiac stress. The major cleavage site takes place at the C-terminus and leads to the release of the Junctophilin-2 N-terminal fragment chain (JP2NT). |
| Function | Junctophilin-2: Membrane-binding protein that provides a structural bridge between the plasma membrane and the sarcoplasmic reticulum and is required for normal excitation-contraction coupling in cardiomyocytes. Provides a structural foundation for functional cross-talk between the cell surface and intracellular Ca(2+) release channels by maintaining the 12-15 nm gap between the sarcolemma and the sarcoplasmic reticulum membranes in the cardiac dyads. Necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions. Junctophilin-2 N-terminal fragment: Transcription repressor required to safeguard against the deleterious effects of cardiac stress. Generated following cleavage of the Junctophilin-2 chain by calpain in response to cardiac stress in cardiomyocytes. Following cleavage and release from the membrane, translocates to the nucleus, binds DNA and represses expression of genes implicated in cell growth and differentiation, hypertrophy, inflammation and fibrosis. Modifies the transcription profile and thereby attenuates pathological remodeling in response to cardiac stress. Probably acts by competing with MEF2 transcription factors and TATA-binding proteins. |
| Protein Name | Junctophilin-2Jp-2Junctophilin Type 2 Cleaved Into - Junctophilin-2 N-Terminal FragmentJp2nt |
| Database Links | |
| Cellular Localisation | Junctophilin-2: Cell MembranePeripheral Membrane ProteinSarcoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinEndoplasmic Reticulum MembraneThe Transmembrane Domain Is Anchored In Sarcoplasmic Reticulum MembraneWhile The N-Terminal Part Associates With The Plasma MembraneIn Heart CellsIt Predominantly Associates Along Z Lines Within MyocytesIn Skeletal MuscleIt Is Specifically Localized At The Junction Of A And I BandsJunctophilin-2 N-Terminal Fragment: NucleusAccumulates In The Nucleus Of Stressed Hearts |
| Alternative Antibody Names | Anti-Junctophilin-2 antibodyAnti-Jp-2 antibodyAnti-Junctophilin Type 2 Cleaved Into - Junctophilin-2 N-Terminal Fragment antibodyAnti-Jp2nt antibodyAnti-JPH2 antibodyAnti-JP2 antibody |
Information sourced from Uniprot.org