• Western blot analysis of extracts of various cell lines, using JAM2 antibody (STJ112019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using JAM2 Rabbit polyclonal antibody (STJ112019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using JAM2 antibody (STJ112019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using JAM2 Rabbit polyclonal antibody (STJ112019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-JAM2 antibody (29-238) (STJ112019)

SKU:
STJ112019

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-JAM2 (29-238) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JAM2
Gene ID: 58494
Uniprot ID: JAM2_HUMAN
Immunogen Region: 29-238
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-238 of human JAM2 (NP_067042.1).
Immunogen Sequence: FSAPKDQQVVTAVEYQEAIL ACKTPKKTVSSRLEWKKLGR SVSFVYYQQTLQGDFKNRAE MIDFNIRIKNVTRSDAGKYR CEVSAPSEQGQNLEEDTVTL EVLVAPAVPSCEVPSSALSG TVVELRCQDKEGNPAPEYTW FKDGIRLLENPRLGSQSTNS SYTMNTKTGTLQFNTVSKLD TGEYSCEARNSVGYRRCPGK RMQVDDLNIS
Tissue Specificity Highly expressed in heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules and also present on the endothelia of other vessels (at protein level). Also expressed in the brain in the caudate nuclei.
Function Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM3 to regulate different cellular processes. Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3. Plays a central role in leukocytes extravasation by facilitating not only transmigration but also tethering and rolling of leukocytes along the endothelium. Tethering and rolling of leukocytes are dependent on the binding by JAM2 of the integrin alpha-4/beta-1. Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation. Also functions as an inhibitory somatodendritic cue that prevents the myelination of non-axonal parts of neurons. During myogenesis, it is involved in myocyte fusion. May also play a role in angiogenesis.
Protein Name Junctional Adhesion Molecule B
Jam-B
Junctional Adhesion Molecule 2
Jam-2
Vascular Endothelial Junction-Associated Molecule
Ve-Jam
Cd Antigen Cd322
Database Links Reactome: R-HSA-202733
Reactome: R-HSA-216083
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Tight Junction
Localized At Tight Junctions Of Both Epithelial And Endothelial Cells
Specifically Localized Within The Somatodendritic Compartment Of Neurons And Excluded From The Axon
Alternative Antibody Names Anti-Junctional Adhesion Molecule B antibody
Anti-Jam-B antibody
Anti-Junctional Adhesion Molecule 2 antibody
Anti-Jam-2 antibody
Anti-Vascular Endothelial Junction-Associated Molecule antibody
Anti-Ve-Jam antibody
Anti-Cd Antigen Cd322 antibody
Anti-JAM2 antibody
Anti-C21orf43 antibody
Anti-VEJAM antibody
Anti-UNQ219 antibody
Anti-PRO245 antibody

Information sourced from Uniprot.org

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