• Immunohistochemistry analysis of IVD in paraffin-embedded mouse kidney using IVD Rabbit polyclonal antibody (STJ117476) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IVD in paraffin-embedded human colon carcinoma using IVD Rabbit polyclonal antibody (STJ117476) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IVD in paraffin-embedded rat liver using IVD Rabbit polyclonal antibody (STJ117476) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using IVD Rabbit polyclonal antibody (STJ117476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.

Anti-IVD antibody (33-200) (STJ117476)

SKU:
STJ117476

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IVD (33-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IVD
Gene ID: 3712
Uniprot ID: IVD_HUMAN
Immunogen Region: 33-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-200 of human IVD (NP_002216.2).
Immunogen Sequence: HSLLPVDDAINGLSEEQRQL RQTMAKFLQEHLAPKAQEID RSNEFKNLREFWKQLGNLGV LGITAPVQYGGSGLGYLEHV LVMEEISRASGAVGLSYGAH SNLCINQLVRNGNEAQKEKY LPKLISGEYIGALAMSEPNA GSDVVSMKLKAEKKGNHYIL NGNKFWIT
Function Catalyzes the conversion of isovaleryl-CoA/3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. To a lesser extent, is also able to catalyze the oxidation of other saturated short-chain acyl-CoA thioesters as pentanoyl-CoA, hexenoyl-CoA and butenoyl-CoA.
Protein Name Isovaleryl-Coa Dehydrogenase - Mitochondrial
Ivd
Butyryl-Coa Dehydrogenase
Database Links Reactome: R-HSA-70895
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Isovaleryl-Coa Dehydrogenase - Mitochondrial antibody
Anti-Ivd antibody
Anti-Butyryl-Coa Dehydrogenase antibody
Anti-IVD antibody

Information sourced from Uniprot.org

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