• Immunofluorescence analysis of U-2 OS cells using ITSN2 Rabbit polyclonal antibody (STJ118952) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ITSN2 Rabbit polyclonal antibody (STJ118952) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ITSN2 Rabbit polyclonal antibody (STJ118952) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ITSN2 Rabbit polyclonal antibody (STJ118952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ITSN2 antibody (500-620) (STJ118952)

SKU:
STJ118952

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ITSN2 (500-620) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITSN2
Gene ID: 50618
Uniprot ID: ITSN2_HUMAN
Immunogen Region: 500-620
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 500-620 of human ITSN2 (NP_006268.2).
Immunogen Sequence: GKHQQISGRLQDVRLKKQTQ KTELEVLDKQCDLEIMEIKQ LQQELQEYQNKLIYLVPEKQ LLNERIKNMQFSNTPDSGVS LLHKKSLEKEELCQRLKEQL DALEKETASKLSEMDSFNNQ L
Tissue Specificity Expressed in melanocytes. Ubiquitous. Isoform 1 is primarily expressed in adult heart and liver.
Function Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). Plays a role in dendrite formation by melanocytes.
Protein Name Intersectin-2
Sh3 Domain-Containing Protein 1b
Sh3p18
Sh3p18-Like Wasp-Associated Protein
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-9013420
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Intersectin-2 antibody
Anti-Sh3 Domain-Containing Protein 1b antibody
Anti-Sh3p18 antibody
Anti-Sh3p18-Like Wasp-Associated Protein antibody
Anti-ITSN2 antibody
Anti-KIAA1256 antibody
Anti-SH3D1B antibody
Anti-SWAP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance