Anti-ITPR3 antibody (Internal) (STJ70840)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ITPR3 (Internal) is suitable for use in ELISA research applications. |
| Applications | Pep-ELISA |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 60kDa band in Human Adipose, Colon, Duodenum, Heart and Liver lysates after 1µg/ml antibody staining. Please note that currently we cannot find |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ITPR3 |
| Gene ID | 3710 |
| Uniprot ID | ITPR3_HUMAN |
| Accession Number | NP_002215.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | DKKERPTDEEGFLH |
| Specificity | No cross-reactivity expected with other types of ITPR |
Additional Info
| Post Translational Modifications | Phosphorylated by AKT1 on serine and/or threonine residues. |
| Function | Inositol 1,4,5-trisphosphate-gated calcium channel that, upon 1D-myo-inositol 1,4,5-trisphosphate binding, transports calcium from the endoplasmic reticulum lumen to cytoplasm, thus releasing the intracellular calcium and therefore participates in cellular calcium ion homeostasis. 1D-myo-inositol 1,4,5-trisphosphate binds to the ligand-free channel without altering its global conformation, yielding the low-energy resting state, then progresses through resting-to preactivated transitions to the higher energy preactivated state, which increases affinity for calcium, promoting binding of the low basal cytosolic calcium at the juxtamembrane domain (JD) site, favoring the transition through the ensemble of high-energy intermediate states along the trajectory to the fully-open activated state. Upon opening, releases calcium in the cytosol where it can bind to the low-affinity cytoplasmic domain (CD) site and stabilizes the inhibited state to terminate calcium release. |
| Protein Name | Inositol 1 -4 -5-Trisphosphate-Gated Calcium Channel Itpr3Ip3 Receptor Isoform 3Ip3r-3Insp3r3Type 3 Inositol 1 -4 -5-Trisphosphate ReceptorType 3 Insp3 Receptor |
| Database Links | Reactome: R-HSA-112043Reactome: R-HSA-114508Reactome: R-HSA-139853Reactome: R-HSA-1489509Reactome: R-HSA-2029485Reactome: R-HSA-2871809Reactome: R-HSA-381676Reactome: R-HSA-4086398Reactome: R-HSA-422356Reactome: R-HSA-5218921Reactome: R-HSA-5578775Reactome: R-HSA-5607763Reactome: R-HSA-9664323Reactome: R-HSA-9717207Reactome: R-HSA-983695 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCytoplasmic VesicleSecretory Vesicle MembraneAlso Localizes At Mitochondria-Associated Membranes (Mams) |
| Alternative Antibody Names | Anti-Inositol 1 -4 -5-Trisphosphate-Gated Calcium Channel Itpr3 antibodyAnti-Ip3 Receptor Isoform 3 antibodyAnti-Ip3r-3 antibodyAnti-Insp3r3 antibodyAnti-Type 3 Inositol 1 -4 -5-Trisphosphate Receptor antibodyAnti-Type 3 Insp3 Receptor antibodyAnti-ITPR3 antibody |
Information sourced from Uniprot.org