• Western blot analysis of extracts of various cell lines, using ITIH3 antibody (STJ116359) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using ITIH3 Rabbit polyclonal antibody (STJ116359) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ITIH3 antibody (35-180) (STJ116359)

SKU:
STJ116359

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ITIH3 (35-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITIH3
Gene ID: 3699
Uniprot ID: ITIH3_HUMAN
Immunogen Region: 35-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-180 of human ITIH3 (NP_002208.3).
Immunogen Sequence: LPEGVANGIEVYSTKINSKV TSRFAHNVVTMRAVNRADTA KEVSFDVELPKTAFITNFTL TIDGVTYPGNVKEKEVAKKQ YEKAVSQGKTAGLVKASGRK LEKFTVSVNVAAGSKVTFEL TYEELLKRHKGKYEMYLKVQ PKQLVK
Post Translational Modifications Heavy chains are linked to bikunin via chondroitin 4-sulfate esterified to the alpha-carboxyl of the C-terminal aspartate after propeptide cleavage.
Function May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Protein Name Inter-Alpha-Trypsin Inhibitor Heavy Chain H3
Iti Heavy Chain H3
Iti-Hc3
Inter-Alpha-Inhibitor Heavy Chain 3
Serum-Derived Hyaluronan-Associated Protein
Shap
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Alternative Antibody Names Anti-Inter-Alpha-Trypsin Inhibitor Heavy Chain H3 antibody
Anti-Iti Heavy Chain H3 antibody
Anti-Iti-Hc3 antibody
Anti-Inter-Alpha-Inhibitor Heavy Chain 3 antibody
Anti-Serum-Derived Hyaluronan-Associated Protein antibody
Anti-Shap antibody
Anti-ITIH3 antibody

Information sourced from Uniprot.org

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