• Confocal immunofluorescence analysis of THP-1 cells using CD11a/LFA-1A/ITGAL Polyclonal Antibody (STJ24250) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen using CD11a/LFA-1A/ITGAL Polyclonal Antibody (STJ24250) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD11a/LFA-1A/ITGAL antibody (STJ24250) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using CD11a/LFA-1A/ITGAL antibody (STJ24250) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using CD11a/LFA-1A/ITGAL antibody (STJ24250) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using CD11a/LFA-1A/ITGAL antibody (STJ24250) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ITGAL antibody (26-1086) (STJ24250)

SKU:
STJ24250

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD11A (26-1086) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGAL
Gene ID: 3683
Uniprot ID: ITAL_HUMAN
Immunogen Region: 26-1086
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-1086 of human CD11a/LFA-1A/ITGAL (NP_001107852.1).
Immunogen Sequence: YNLDVRGARSFSPPRAGRHF GYRVLQVGNGVIVGAPGEGN STGSLYQCQSGTGHCLPVTL RGSNYTSKYLGMTLATDPTD GSILFAAVQFSTSYKTEFDF SDYVKRKDPDALLKHVKHML LLTNTFGAINYVATEVFREE LGARPDATKVLIIITDGEAT DSGNIDAAKDIIRYIIGIGK HFQTKESQETLHKFASKPAS EFVKILDTFEKLKDLFTELQ KKIYVIEGTSKQDLTSFNM
Tissue Specificity Leukocytes.
Post Translational Modifications In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for activation by some physiological agents.
Function Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R. Integrin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15.the interaction is mediated by ITGAL. Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
Protein Name Integrin Alpha-L
Cd11 Antigen-Like Family Member A
Leukocyte Adhesion Glycoprotein Lfa-1 Alpha Chain
Lfa-1a
Leukocyte Function-Associated Molecule 1 Alpha Chain
Cd Antigen Cd11a
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-6798695
Reactome: R-HSA-8949275
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Integrin Alpha-L antibody
Anti-Cd11 Antigen-Like Family Member A antibody
Anti-Leukocyte Adhesion Glycoprotein Lfa-1 Alpha Chain antibody
Anti-Lfa-1a antibody
Anti-Leukocyte Function-Associated Molecule 1 Alpha Chain antibody
Anti-Cd Antigen Cd11a antibody
Anti-ITGAL antibody
Anti-CD11A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance