• Western blot analysis of extracts of various cell lines, using ITGAE antibody (STJ111975) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using ITGAE antibody (STJ111975) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ITGAE antibody (19-200) (STJ111975)

SKU:
STJ111975

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ITGAE (19-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGAE
Gene ID: 3682
Uniprot ID: ITAE_HUMAN
Immunogen Region: 19-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-200 of human ITGAE (NP_002199.3).
Immunogen Sequence: FNVDVARPWLTPKGGAPFVL SSLLHQDPSTNQTWLLVTSP RTKRTPGPLHRCSLVQDEIL CHPVEHVPIPKGRHRGVTVV RSHHGVLICIQVLVRRPHSL SSELTGTCSLLGPDLRPQAQ ANFFDLENLLDPDARVDTGD CYSNKEGGGEDDVNTARQRR ALEKEEEEDKEEEEDEEEEE AG
Tissue Specificity Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
Function Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
Protein Name Integrin Alpha-E
Hml-1 Antigen
Integrin Alpha-Iel
Mucosal Lymphocyte 1 Antigen
Cd Antigen Cd103 Cleaved Into - Integrin Alpha-E Light Chain - Integrin Alpha-E Heavy Chain
Database Links Reactome: R-HSA-216083
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Integrin Alpha-E antibody
Anti-Hml-1 Antigen antibody
Anti-Integrin Alpha-Iel antibody
Anti-Mucosal Lymphocyte 1 Antigen antibody
Anti-Cd Antigen Cd103 Cleaved Into - Integrin Alpha-E Light Chain - Integrin Alpha-E Heavy Chain antibody
Anti-ITGAE antibody

Information sourced from Uniprot.org

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