• Immunohistochemistry analysis of paraffin-embedded mouse testis using Integrin alpha 4 (ITGA4/CD49d) (ITGA4/CD49d) Rabbit monoclonal antibody (STJ11101987) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Integrin alpha 4 (ITGA4/CD49d) (ITGA4/CD49d) Rabbit monoclonal antibody (STJ11101987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ITGA4 antibody (933-1032) [S7MR] (STJ11101987)

SKU:
STJ11101987

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Integrin alpha 4 (933-1032) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGA4
Gene ID: 3676
Uniprot ID: ITA4_HUMAN
Immunogen Region: 933-1032
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 933-1032 of human Integrin alpha 4 (ITGA4/CD49d) (P13612).
Immunogen Sequence: ALKFEIRATGFPEPNPRVIE LNKDENVAHVLLEGLHHQRP KRYFTIVIISSSLLLGLIVL LLISYVMWKAGFFKRQYKSI LQEENRRDSWSYINSKSNDD
Post Translational Modifications Phosphorylation on Ser-1027 inhibits PXN binding.
Function Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.
Protein Name Integrin Alpha-4
Cd49 Antigen-Like Family Member D
Integrin Alpha-Iv
Vla-4 Subunit Alpha
Cd Antigen Cd49d
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-8949275
Reactome: R-HSA-9679191
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Integrin Alpha-4 antibody
Anti-Cd49 Antigen-Like Family Member D antibody
Anti-Integrin Alpha-Iv antibody
Anti-Vla-4 Subunit Alpha antibody
Anti-Cd Antigen Cd49d antibody
Anti-ITGA4 antibody
Anti-CD49D antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance