• Immunohistochemistry analysis of paraffin-embedded human lung cancer (left) and cervical carcinoma (right) , showing nuclear localization with DAB staining using Islet-1 monoclonal antibody.
  • Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using Islet-1 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
  • Western blot analysis using Islet-1 monoclonal antibody against full-length ISL1 (aa1-349) -hIgGFc transfected HEK293 cell lysate (1).

Anti-ISL1 antibody [1H9] (STJ98184)

SKU:
STJ98184

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Insulin gene enhancer protein ISL-1 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: 1H9
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ISL1
Gene ID: 3670
Uniprot ID: ISL1_HUMAN
Specificity: Islet-1 Monoclonal Antibody detects endogenous levels of Islet-1 protein.
Immunogen: Purified recombinant fragment of human Islet-1 expressed in E. Coli.
Post Translational Modifications Ubiquitinated probably by WWP1 E3 ubiquitin ligase.ubiquitination is followed by protein degradation. Phosphorylated.
Function DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart.
Protein Name Insulin Gene Enhancer Protein Isl-1
Islet-1
Database Links Reactome: R-HSA-400511
Reactome: R-HSA-9010553
Reactome: R-HSA-9733709
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Insulin Gene Enhancer Protein Isl-1 antibody
Anti-Islet-1 antibody
Anti-ISL1 antibody

Information sourced from Uniprot.org

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