• Western blot analysis of various lysates, using IRS2 rabbit polyclonal antibody (STJ110254) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human liver using IRS2 rabbit polyclonal antibody (STJ110254) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using IRS2 rabbit polyclonal antibody (STJ110254) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using IRS2 rabbit polyclonal antibody (STJ110254) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of SH-SY5Y cells using IRS2 rabbit polyclonal antibody (STJ110254) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IRS2 antibody (1238-1338) (STJ110254)

SKU:
STJ110254

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IRS2 (1238-1338) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRS2
Gene ID: 8660
Uniprot ID: IRS2_HUMAN
Immunogen Region: 1238-1338
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1238-1338 of human IRS2 (NP_003740.2).
Immunogen Sequence: PMRRETSAGFQNGLNYIAID VREEPGLPPQPQPPPPPLPQ PGDKSSWGRTRSLGGLISAV GVGSTGGGCGGPGPGALPPA NTYASIDFLSHHLKEATIVK E
Function May mediate the control of various cellular processes by insulin.
Protein Name Insulin Receptor Substrate 2
Irs-2
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-112399
Reactome: R-HSA-112412
Reactome: R-HSA-1257604
Reactome: R-HSA-1266695
Reactome: R-HSA-198203
Reactome: R-HSA-2219530
Reactome: R-HSA-2428928
Reactome: R-HSA-2586552
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-74713
Reactome: R-HSA-74749
Reactome: R-HSA-8853659
Reactome: R-HSA-9006335
Reactome: R-HSA-9027276
Reactome: R-HSA-9027277
Reactome: R-HSA-9027283
Reactome: R-HSA-9027284
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Insulin Receptor Substrate 2 antibody
Anti-Irs-2 antibody
Anti-IRS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance