| Short Description : | Goat polyclonal antibody anti-IRF2BP1 (109-122) is suitable for use in ELISA and Western Blot research applications. |
| Applications: | Pep-ELISA/WB |
| Host: | Goat |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75kDa band observed in lysates of cell line HepG2 and 80kDa in nuclear lysates of cell line NIH3T3 (calculated MW of 61.7kDa according to NP_056464.1). Recommended concentration |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | IRF2BP1 |
| Gene ID: | 26145 |
| Uniprot ID: | I2BP1_HUMAN |
| Accession Number: | NP_056464.1 |
| Immunogen Region: | 109-122 |
| Immunogen Sequence: | QDRYDRATSSGRLP |
| Protein Name | Interferon Regulatory Factor 2-Binding Protein 1Irf-2-Binding Protein 1Irf-2bp1Probable E3 Ubiquitin-Protein Ligase Irf2bp1Probable Ring-Type E3 Ubiquitin Transferase Irf2bp1 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Interferon Regulatory Factor 2-Binding Protein 1 antibodyAnti-Irf-2-Binding Protein 1 antibodyAnti-Irf-2bp1 antibodyAnti-Probable E3 Ubiquitin-Protein Ligase Irf2bp1 antibodyAnti-Probable Ring-Type E3 Ubiquitin Transferase Irf2bp1 antibodyAnti-IRF2BP1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and UBE2S knockout (KO) 293T cells, using [KO Validated] UBE2S Rabbit polyclonal antibody (STJ11100037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89109/357563/STJ11100037_1__21240.1713121689.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and UBE2S knockout (KO) 293T cells, using [KO Validated] UBE2S Rabbit polyclonal antibody (STJ11100037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
