Anti-IQGAP1 antibody (Internal) (STJ72002)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-IQGAP1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 200kDa band observed in Human Kidney lysates (calculated MW of 189kDa according to NP_003861.1). Recommended concentration: 1-3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | IQGAP1 |
| Gene ID | 8826 |
| Uniprot ID | IQGA1_HUMAN |
| Accession Number | NP_003861.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | KRQSGQTDPLQKEE |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. |
| Function | Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane. Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth. May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest. |
| Protein Name | Ras Gtpase-Activating-Like Protein Iqgap1P195 |
| Database Links | Reactome: R-HSA-373753Reactome: R-HSA-381676Reactome: R-HSA-5626467Reactome: R-HSA-5674135Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8980692Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013406Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9649948Reactome: R-HSA-9656223 |
| Cellular Localisation | Cell MembraneNucleusCytoplasmCell CortexApical Cell MembraneBasolateral Cell MembraneSubcellular Distribution Is Regulated By The Cell CycleNuclear Levels Increase At G1/S Phase |
| Alternative Antibody Names | Anti-Ras Gtpase-Activating-Like Protein Iqgap1 antibodyAnti-P195 antibodyAnti-IQGAP1 antibodyAnti-KIAA0051 antibody |
Information sourced from Uniprot.org