• Immunofluorescence analysis of L929 cells using IPO4 Rabbit polyclonal antibody (STJ118049) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using IPO4 Rabbit polyclonal antibody (STJ118049) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using IPO4 Rabbit polyclonal antibody (STJ118049) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using IPO4 Rabbit polyclonal antibody (STJ118049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IPO4 antibody (728-1081) (STJ118049)

SKU:
STJ118049

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IPO4 (728-1081) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IPO4
Gene ID: 79711
Uniprot ID: IPO4_HUMAN
Immunogen Region: 728-1081
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 728-1081 of human IPO4 (NP_078934.3).
Immunogen Sequence: LHKACQSCPSEPNTAALQAA LARVVPSYMQAVNRERERQV VMAVLEALTGVLRSCGTLTL KPPGRLAELCGVLKAVLQRK TACQDTDEEEEEEDDDQAEY DAMLLEHAGEAIPALAAAAG GDSFAPFFAGFLPLLVCKTK QGCTVAEKSFAVGTLAETIQ GLGAASAQFVSRLLPVLLST AQEADPEVRSNAIFGMGVLA EHGGHPAQEHFPKLLGLLFP LLARERHDRVRDNICGALA
Function Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of the histone H3-H4 dimer when in complex with ASF1 (ASF1A or ASF1B). Mediates the ligand-independent nuclear import of vitamin D receptor (VDR). In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Protein Name Importin-4
Imp4
Importin-4b
Imp4b
Ran-Binding Protein 4
Ranbp4
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Importin-4 antibody
Anti-Imp4 antibody
Anti-Importin-4b antibody
Anti-Imp4b antibody
Anti-Ran-Binding Protein 4 antibody
Anti-Ranbp4 antibody
Anti-IPO4 antibody
Anti-IMP4B antibody
Anti-RANBP4 antibody

Information sourced from Uniprot.org

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