• Western blot analysis of extracts of Rat lung, using Involucrin antibody (STJ11102752) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded rat skin using Involucrin rabbit monoclonal antibody (STJ11102752) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Involucrin rabbit monoclonal antibody (STJ11102752) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse skin using Involucrin rabbit monoclonal antibody (STJ11102752) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Involucrin in paraffin-embedded human cervix cancer tissue using Involucrin rabbit monoclonal antibody (STJ11102752) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-Involucrin antibody (100-200) [S2MR] (STJ11102752)

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STJ11102752

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Involucrin (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IVL
Gene ID: 3713
Uniprot ID: INVO_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Involucrin (P07476).
Immunogen Sequence: KAENPEQQLKQEKTQRDQQL NKQLEEEKKLLDQQLDQELV KRDEQLGMKKEQLLELPEQQ EGHLKHLEQQEGQLKHPEQQ EGQLELPEQQEGQLELPEQQ E
Tissue Specificity Keratinocytes of epidermis and other stratified squamous epithelia.
Post Translational Modifications Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Function Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Protein Name Involucrin
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Constituent Of The Scaffolding Of The Cornified Envelope
Alternative Antibody Names Anti-Involucrin antibody
Anti-IVL antibody

Information sourced from Uniprot.org

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