Anti-INTS13 antibody (200-300 aa) [S4957RM] (STJ11104957)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Integrator Complex Subunit 13 (200-300 aa) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4957RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:10000-1:80000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | INTS13 |
| Gene ID | 55726 |
| Uniprot ID | INT13_HUMAN |
| Immunogen Region | 200-300 aa |
| Immunogen Sequence | QKCELVLIHTYPVGEDSLVS DRSKKELSPVLTSEVHSVRA GRHLATKLNILVQQHFDLAS TTITNIPMKEEQHANTSANY DVELLHHKDAHVDFLKSGDS H |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human ASUN/INTS13. (NP_060634.2). |
Additional Info
| Tissue Specificity | Widely expressed. Tends to be up-regulated in seminomas compared to normal testis. |
| Function | Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Within the integrator complex, INTS13 is part of the integrator tail module and acts as a platform for the recruitment of transcription factors at promoters. At prophase, mediates recruitment of cytoplasmic dynein to the nuclear envelope, a step important for proper centrosome-nucleus coupling. At G2/M phase, may be required for proper spindle formation and execution of cytokinesis. |
| Protein Name | Integrator Complex Subunit 13Cell Cycle Regulator Mat89bb HomologGerm Cell Tumor 1Protein Asunder HomologSarcoma Antigen Ny-Sar-95 |
| Database Links | Reactome: R-HSA-6807505 |
| Cellular Localisation | NucleusCytoplasmNuclear Location Is Required For Recruitment Of Dynein Motors To Nuclear Envelope At G2/M |
| Alternative Antibody Names | Anti-Integrator Complex Subunit 13 antibodyAnti-Cell Cycle Regulator Mat89bb Homolog antibodyAnti-Germ Cell Tumor 1 antibodyAnti-Protein Asunder Homolog antibodyAnti-Sarcoma Antigen Ny-Sar-95 antibodyAnti-INTS13 antibodyAnti-ASUN antibodyAnti-C12orf11 antibodyAnti-GCT1 antibody |
Information sourced from Uniprot.org