Anti-INTS12 antibody (1-230 aa) [SRM5280] (STJ11107182)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal INTS12 (1-230 aa) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5280 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | INTS12 |
| Gene ID | 57117 |
| Uniprot ID | INT12_HUMAN |
| Immunogen Region | 1-230 aa |
| Immunogen Sequence | MAATVNLELDPIFLKALGFL HSKSKDSAEKLKALLDESLA RGIDSSYRPSQKDVEPPKIS STKNISIKQEPKISSSLPSG NNNGKVLTTEKVKKEAEKRP ADKMKSDITEGVDIPKKPRL EKPETQSSPITVQSSKDLPM ADLSSFEETSADDFAMEMGL ACVVCRQMMVASGNQLVECQ ECHNLYHRDCHKPQVTDKEA NDPRLVWYCARCTRQMKRMA QKTQKPPQKP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human INTS12 (NP_065128.2). |
Additional Info
| Post Translational Modifications | Dephosphorylated at Ser-128 by the PNUTS-PP1 complex, promoting RNA polymerase II transcription pause-release. |
| Function | Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex. |
| Protein Name | Integrator Complex Subunit 12Int12Phd Finger Protein 22 |
| Database Links | Reactome: R-HSA-6807505 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Integrator Complex Subunit 12 antibodyAnti-Int12 antibodyAnti-Phd Finger Protein 22 antibodyAnti-INTS12 antibodyAnti-PHF22 antibodyAnti-SBBI22 antibody |
Information sourced from Uniprot.org