• Western blot analysis of various lysates, using Insulin antibody (STJ24210) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Insulin rabbit polyclonal antibody (STJ24210) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of human pancreas cells using Insulin rabbit polyclonal antibody (STJ24210) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse pancreas cells using Insulin rabbit polyclonal antibody (STJ24210) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat pancreas cells using Insulin rabbit polyclonal antibody (STJ24210) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-INS antibody (1-110) (STJ24210)

SKU:
STJ24210

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INS
Gene ID: 3630
Uniprot ID: INS_HUMAN
Immunogen Region: 1-110
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS (NP_000198.1).
Immunogen Sequence: MALWMRLLPLLALLALWGPD PAAAFVNQHLCGSHLVEALY LVCGERGFFYTPKTRREAED LQVGQVELGGGPGAGSLQPL ALEGSLQKRGIVEQCCTSIC SLYQLENYCN
Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Protein Name Insulin Cleaved Into - Insulin B Chain - Insulin A Chain
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-264876
Reactome: R-HSA-422085
Reactome: R-HSA-422356
Reactome: R-HSA-6807878
Reactome: R-HSA-6811558
Reactome: R-HSA-74713
Reactome: R-HSA-74749
Reactome: R-HSA-74751
Reactome: R-HSA-74752
Reactome: R-HSA-77387
Reactome: R-HSA-9615017
Reactome: R-HSA-9768919
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin Cleaved Into - Insulin B Chain - Insulin A Chain antibody
Anti-INS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance