• Immunofluorescence analysis of L929 cells using INPP5E Rabbit polyclonal antibody (STJ119776) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using INPP5E Rabbit polyclonal antibody (STJ119776) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from SH-SY5Y cells, using INPP5E Rabbit polyclonal antibody (STJ119776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-INPP5E antibody (510-630) (STJ119776)

SKU:
STJ119776

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-INPP5E (510-630) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INPP5E
Gene ID: 56623
Uniprot ID: INP5E_HUMAN
Immunogen Region: 510-630
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 510-630 of human INPP5E (NP_063945.2).
Immunogen Sequence: LIREMRKGSIFKGFQEPDIH FLPSYKFDIGKDTYDSTSKQ RTPSYTDRVLYRSRHKGDIC PVSYSSCPGIKTSDHRPVYG LFRVKVRPGRDNIPLAAGKF DRELYLLGIKRRISKEIQRQ Q
Tissue Specificity Detected in brain, heart, pancreas, testis and spleen.
Function Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Specific for lipid substrates, inactive towards water soluble inositol phosphates. Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability.
Protein Name Phosphatidylinositol Polyphosphate 5-Phosphatase Type Iv
72 Kda Inositol Polyphosphate 5-Phosphatase
Inositol Polyphosphate-5-Phosphatase E
Phosphatidylinositol 4 -5-Bisphosphate 5-Phosphatase
Phosphatidylinositol-3 -4 -5-Trisphosphate 5-Phosphatase
Database Links Reactome: R-HSA-1660514
Reactome: R-HSA-5624958
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Axoneme
Golgi Apparatus
Golgi Stack Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Membrane
Cell Projection
Ruffle
Nucleus
Peripheral Membrane Protein Associated With Golgi Stacks
Alternative Antibody Names Anti-Phosphatidylinositol Polyphosphate 5-Phosphatase Type Iv antibody
Anti-72 Kda Inositol Polyphosphate 5-Phosphatase antibody
Anti-Inositol Polyphosphate-5-Phosphatase E antibody
Anti-Phosphatidylinositol 4 -5-Bisphosphate 5-Phosphatase antibody
Anti-Phosphatidylinositol-3 -4 -5-Trisphosphate 5-Phosphatase antibody
Anti-INPP5E antibody

Information sourced from Uniprot.org

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