• Western blot analysis of extracts of various cell lines, using Alpha Internexin antibody (STJ24201) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-INA antibody (13-300) (STJ24201)

SKU:
STJ24201

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-INA (13-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INA
Gene ID: 9118
Uniprot ID: AINX_HUMAN
Immunogen Region: 13-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 13-300 of human Alpha Internexin (Q16352).
Immunogen Sequence: SSYRKVFGDGSRLSARLSGA GGAGGFRSQSLSRSNVASSA ACSSASSLGLGLAYRRPPAS DGLDLSQAAARTNEYKIIRT NEKEQLQGLNDRFAVFIEKV HQLETQNRALEAELAALRQR HAEPSRVGELFQRELRDLRA QLEEASSARSQALLERDGLA EEVQRLRARCEEESRGREGA ERALKAQQRDVDGATLARLD LEKKVESLLDELAFVRQVHD EEVAELLATLQASSQAAAE
Tissue Specificity Found predominantly in adult CNS.
Post Translational Modifications O-glycosylated.
Function Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks.
Protein Name Alpha-Internexin
Alpha-Inx
66 Kda Neurofilament Protein
Nf-66
Neurofilament-66
Neurofilament 5
Alternative Antibody Names Anti-Alpha-Internexin antibody
Anti-Alpha-Inx antibody
Anti-66 Kda Neurofilament Protein antibody
Anti-Nf-66 antibody
Anti-Neurofilament-66 antibody
Anti-Neurofilament 5 antibody
Anti-INA antibody
Anti-NEF5 antibody

Information sourced from Uniprot.org

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