• Immunohistochemistry analysis of paraffin-embedded mouse brain using ILK Antibody (STJ24198) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ILK Antibody (STJ24198) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ILK antibody (STJ24198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ILK antibody (1-260) (STJ24198)

SKU:
STJ24198

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ILK (1-260) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ILK
Gene ID: 3611
Uniprot ID: ILK_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ILK (NP_001014795.1).
Immunogen Sequence: MDDIFTQCREGNAVAVRLWL DNTENDLNQGDDHGFSPLHW ACREGRSAVVEMLIMRGARI NVMNRGDDTPLHLAASHGHR DIVQKLLQYKADINAVNEHG NVPLHYACFWGQDQVAEDLV ANGALVSICNKYGEMPVDKA KAPLRELLRERAEKMGQNLN RIPYKDTFWKGTTRTRPRNG TLNKHSGIDFKQLNFLTKLN ENHSGELWKGRWQGNDIVVK VLKVRDWSTRKSRDFNEEC
Tissue Specificity Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Post Translational Modifications Autophosphorylated on serine residues.
Function Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Regulates cell motility by forming a complex with PARVB. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Protein Name Integrin-Linked Protein Kinase
59 Kda Serine/Threonine-Protein Kinase
Beta-Integrin-Linked Kinase
Ilk-1
Ilk-2
P59ilk
Database Links Reactome: R-HSA-446343
Reactome: R-HSA-446353
Cellular Localisation Cell Junction
Focal Adhesion
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Lamellipodium
Cytoplasm
Myofibril
Sarcomere
Alternative Antibody Names Anti-Integrin-Linked Protein Kinase antibody
Anti-59 Kda Serine/Threonine-Protein Kinase antibody
Anti-Beta-Integrin-Linked Kinase antibody
Anti-Ilk-1 antibody
Anti-Ilk-2 antibody
Anti-P59ilk antibody
Anti-ILK antibody
Anti-ILK1 antibody
Anti-ILK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance