• Biotinylated STJ71178 (0. 1µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ILF2/NF45, Biotinylated antibody (Internal) {Biotin} (STJ73229)

SKU:
STJ73229-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/PAR-CLIP
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ILF2/NF45, Biotinylated (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and PAR-CLImmunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ILF2
Gene ID: 3608
Uniprot ID: ILF2_HUMAN
Immunogen Region: Internal
Accession Number: NP_004506.2; NP_001254738.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_004506.2; NP_001254738.1).
Immunogen Sequence: EKAYEKPPEKKEG.
Function Chromatin-interacting protein that forms a stable heterodimer with interleukin enhancer-binding factor 3/ILF3 and plays a role in several biological processes including transcription, innate immunity or cell growth. Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus. Together with ILF3, forms an RNA-binding complex that is required for mitotic progression and cytokinesis by regulating the expression of a cluster of mitotic genes. Mechanistically, competes with STAU1/STAU2-mediated mRNA decay. Also plays a role in the inhibition of various viruses including Japanese encephalitis virus or enterovirus 71. (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
Protein Name Interleukin Enhancer-Binding Factor 2
Nuclear Factor Of Activated T-Cells 45 Kda
Database Links Reactome: R-HSA-6798695
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Interleukin Enhancer-Binding Factor 2 antibody
Anti-Nuclear Factor Of Activated T-Cells 45 Kda antibody
Anti-ILF2 antibody
Anti-NF45 antibody
Anti-PRO3063 antibody

Information sourced from Uniprot.org

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