| Short Description : | Rabbit polyclonal CD127 (21-239) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IL7R |
| Gene ID: | 3575 |
| Uniprot ID: | IL7RA_HUMAN |
| Immunogen Region: | 21-239 |
| Immunogen Sequence: | ESGYAQNGDLEDAELDDYSF SCYSQLEVNGSQHSLTCAFE DPDVNITNLEFEICGALVEV KCLNFRKLQEIYFIETKKFL LIGKSNICVKVGEKSLTCKK IDLTTIVKPEAPFDLSVVYR EGANDFVVTFNTSHLQKKYV KVLMHDVAYRQEKDENKWTH VNLSSTKLTLLQRKLQPAAM YEIKVRSIPDHYFKGFWSEW SPSYYFRTPEINNSSGEMD |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-239 of human CD127/IL7R (NP_002176.2). |
| Post Translational Modifications | N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form. Ubiquitinated by MARCHF8.leading to lysosomal degradation. |
| Function | Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). |
| Protein Name | Interleukin-7 Receptor Subunit AlphaIl-7 Receptor Subunit AlphaIl-7r Subunit AlphaIl-7r-AlphaIl-7raCdw127Cd Antigen Cd127 |
| Database Links | Reactome: R-HSA-1266695Reactome: R-HSA-8856825Reactome: R-HSA-8856828 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 3: Cell MembraneIsoform 4: Secreted |
| Alternative Antibody Names | Anti-Interleukin-7 Receptor Subunit Alpha antibodyAnti-Il-7 Receptor Subunit Alpha antibodyAnti-Il-7r Subunit Alpha antibodyAnti-Il-7r-Alpha antibodyAnti-Il-7ra antibodyAnti-Cdw127 antibodyAnti-Cd Antigen Cd127 antibodyAnti-IL7R antibody |
Information sourced from Uniprot.org
![C57BL/6 murine splenocytes were stained with Anti-Mouse CD127 Monoclonal Antibody (APC Conjugated)[Used at 0.2 µg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/145109/291775/STJA0000256_1__06608.1631205561.jpg?c=1 "C57BL/6 murine splenocytes were stained with Anti-Mouse CD127 Monoclonal Antibody (APC Conjugated)[Used at 0.2 µg/10<sup>6</sup> cells dilution] (filled gray histogram). Unstained splenocytes (empty black histogram) were used as control.")
