• Immunofluorescence analysis of U2OS cells using IL6ST Rabbit polyclonal antibody (STJ11100009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using IL6ST Rabbit polyclonal antibody (STJ11100009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using IL6ST Rabbit polyclonal antibody (STJ11100009) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL6ST antibody (751-918) (STJ11100009)

SKU:
STJ11100009

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL6ST (751-918) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL6ST
Gene ID: 3572
Uniprot ID: IL6RB_HUMAN
Immunogen Region: 751-918
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 751-918 of human IL6ST (NP_002175.2).
Immunogen Sequence: QNTSSTVQYSTVVHSGYRHQ VPSVQVFSRSESTQPLLDSE ERPEDLQLVDHVDGGDGILP RQQYFKQNCSQHESSPDISH FERSKQVSSVNEEDFVRLKQ QISDHISQSCGSGQMKMFQE VSAADAFGPGTEGQVERFET VGMEAATDEGMPKSYLPQTV RQGGYMPQ
Tissue Specificity Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. Isoform 2: Expressed in blood serum (at protein level).
Post Translational Modifications Phosphorylation of Ser-782 down-regulates cell surface expression. Heavily N-glycosylated. Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding.
Function Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop. Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3. Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism. Has a role in embryonic development. Essential for survival of motor and sensory neurons and for differentiation of astrocytes. Required for expression of TRPA1 in nociceptive neurons. Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation. Required for normal trabecular bone mass and cortical bone composition. Isoform 2: Binds to the soluble IL6:sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response. Also blocks IL11 cluster signaling through IL11R.
Protein Name Interleukin-6 Receptor Subunit Beta
Il-6 Receptor Subunit Beta
Il-6r Subunit Beta
Il-6r-Beta
Il-6rb
Cdw130
Interleukin-6 Signal Transducer
Membrane Glycoprotein 130
Gp130
Oncostatin-M Receptor Subunit Alpha
Cd Antigen Cd130
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-110056 P40189-1
Reactome: R-HSA-112411 P40189-1
Reactome: R-HSA-6788467
Reactome: R-HSA-8984722
Reactome: R-HSA-9020956
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Interleukin-6 Receptor Subunit Beta antibody
Anti-Il-6 Receptor Subunit Beta antibody
Anti-Il-6r Subunit Beta antibody
Anti-Il-6r-Beta antibody
Anti-Il-6rb antibody
Anti-Cdw130 antibody
Anti-Interleukin-6 Signal Transducer antibody
Anti-Membrane Glycoprotein 130 antibody
Anti-Gp130 antibody
Anti-Oncostatin-M Receptor Subunit Alpha antibody
Anti-Cd Antigen Cd130 antibody
Anti-IL6ST antibody

Information sourced from Uniprot.org

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