Anti-IL36A antibody (1-158) (STJ110506)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IL36A (1-158) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IL36A |
| Gene ID | 27179 |
| Uniprot ID | IL36A_HUMAN |
| Immunogen Region | 1-158 |
| Immunogen Sequence | MEKALKIDTPQQGSIQDINH RVWVLQDQTLIAVPRKDRMS PVTIALISCRHVETLEKDRG NPIYLGLNGLNLCLMCAKVG DQPTLQLKEKDIMDLYNQPE PVKSFLFYHSQSGRNSTFES VAFPGWFIAVSSEGGCPLIL TQELGKANTTDFGLTMLF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human IL36A (NP_055255.1). |
Additional Info
| Tissue Specificity | Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin. |
| Post Translational Modifications | N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). |
| Function | Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response.similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of pro-inflammatory cytokines such as TNF-alpha, IL-8 and IL-6. In cultured monocytes up-regulates expression of IL-1A, IL-1B and IL-6. In myeloid dendritic cells involved in cell maturation by up-regulating surface expression of CD83, CD86 and HLA-DR. In monocyte-derived dendritic cells facilitates dendritic cell maturation and drives T-cell proliferation. May play a role in pro-inflammatory effects in the lung. |
| Protein Name | Interleukin-36 AlphaFil1 EpsilonInterleukin-1 EpsilonIl-1 EpsilonInterleukin-1 Family Member 6Il-1f6 |
| Database Links | Reactome: R-HSA-9014826 |
| Cellular Localisation | CytoplasmSecretedThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Antibody Names | Anti-Interleukin-36 Alpha antibodyAnti-Fil1 Epsilon antibodyAnti-Interleukin-1 Epsilon antibodyAnti-Il-1 Epsilon antibodyAnti-Interleukin-1 Family Member 6 antibodyAnti-Il-1f6 antibodyAnti-IL36A antibodyAnti-FIL1E antibodyAnti-IL1E antibodyAnti-IL1F6 antibody |
Information sourced from Uniprot.org