• Western blot analysis of extracts of A-431 cells, using IL1RA antibody (STJ24179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of human skin cancer using IL1RA rabbit polyclonal antibody (STJ24179) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A431 cells using IL1RA rabbit polyclonal antibody (STJ24179) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using IL1RA rabbit polyclonal antibody (STJ24179) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL1RN antibody (24-177) (STJ24179)

SKU:
STJ24179

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL1RN (24-177) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL1RN
Gene ID: 3557
Uniprot ID: IL1RA_HUMAN
Immunogen Region: 24-177
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-177 of human IL1RA (NP_776214.1).
Immunogen Sequence: ICRPSGRKSSKMQAFRIWDV NQKTFYLRNNQLVAGYLQGP NVNLEEKIDVVPIEPHALFL GIHGGKMCLSCVKSGDETRL QLEAVNITDLSENRKQDKRF AFIRSDSGPTTSFESAACPG WFLCTAMEADQPVSLTNMPD EGVMVTKFYFQEDE
Tissue Specificity The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Function Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
Protein Name Interleukin-1 Receptor Antagonist Protein
Il-1rn
Il-1ra
Irap
Icil-1ra
Il1 Inhibitor
Anakinra
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-9020702
Cellular Localisation Isoform 1: Secreted
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Isoform 4: Cytoplasm
Alternative Antibody Names Anti-Interleukin-1 Receptor Antagonist Protein antibody
Anti-Il-1rn antibody
Anti-Il-1ra antibody
Anti-Irap antibody
Anti-Icil-1ra antibody
Anti-Il1 Inhibitor antibody
Anti-Anakinra antibody
Anti-IL1RN antibody
Anti-IL1F3 antibody
Anti-IL1RA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance