Anti-IL1Beta antibody (100-269) [S5759RM] (STJ11105759)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal IL1Beta (100-269) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5759RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Il1b |
| Gene ID | 16176 |
| Uniprot ID | IL1B_MOUSE |
| Immunogen Region | 100-269 |
| Immunogen Sequence | EPILCDSWDDDDNLLVCDVP IRQLHYRLRDEQQKSLVLSD PYELKALHLNGQNINQQVIF SMSFVQGEPSNDKIPVALGL KGKNLYLSCVMKDGTPTLQL ESVDPKQYPKKKMEKRFVFN KIEVKSKVEFESAEFPNWYI STSQAEHKPVFLGNNSGQDI IDFTMESVSS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 100-269 of mouse IL1 Beta (P10749). |
Additional Info
| Tissue Specificity | Expressed in activated macrophages (at protein level). |
| Function | Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-1 BetaIl-1 Beta |
| Database Links | Reactome: R-MMU-448706Reactome: -MMU-5620971Reactome: -MMU-5660668Reactome: -MMU-9020702 |
| Cellular Localisation | CytoplasmCytosolSecretedLysosomeExtracellular ExosomeThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsSuch As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 InflammasomeCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Antibody Names | Anti-Interleukin-1 Beta antibodyAnti-Il-1 Beta antibodyAnti-Il1b antibody |
Information sourced from Uniprot.org