Anti-IL18 antibody (1-193 aa) [SRM5148] (STJ11106987)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal IL18 (1-193 aa) antibody for use in WB, IHC-P, IF and ICC in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5148 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200 |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
Target Information
| Gene Symbol | IL18 |
| Gene ID | 3606 |
| Uniprot ID | IL18_HUMAN |
| Immunogen Region | 1-193 aa |
| Immunogen Sequence | MAAEPVEDNCINFVAMKFID NTLYFIAEDDENLESDYFGK LESKLSVIRNLNDQVLFIDQ GNRPLFEDMTDSDCRDNAPR TIFIISMYKDSQPRGMAVTI SVKCEKISTLSCENKIISFK EMNPPDNIKDTKSDIIFFQR SVPGHDNKMQFESSSYEGYF LACEKERDLFKLILKKEDEL GDRSIMFTVQNED |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human IL18 (NP_001553.1) |
Additional Info
| Tissue Specificity | Isoform 2: Expressed in ovarian carcinoma but undetectable in normal ovarian epithelial cells. Resistant to proteolytic activation by caspase-1 and -4. |
| Post Translational Modifications | The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form. The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor. Processing by CASP1, CASP4 or CASP5 induces conformational changes to generate critical receptor-binding sites. The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, cleavage by CASP3 inactivates IL18. |
| Function | Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-18Il-18IboctadekinInterferon Gamma-Inducing FactorIfn-Gamma-Inducing FactorInterleukin-1 GammaIl-1 Gamma |
| Database Links | Reactome: R-HSA-448706Reactome: R-HSA-5620971Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9012546 Q14116-2Reactome: R-HSA-9660826 |
| Cellular Localisation | CytoplasmCytosolSecretedThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1Casp4 Or Casp5 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Antibody Names | Anti-Interleukin-18 antibodyAnti-Il-18 antibodyAnti-Iboctadekin antibodyAnti-Interferon Gamma-Inducing Factor antibodyAnti-Ifn-Gamma-Inducing Factor antibodyAnti-Interleukin-1 Gamma antibodyAnti-Il-1 Gamma antibodyAnti-IL18 antibodyAnti-IGIF antibodyAnti-IL1F4 antibody |
Information sourced from Uniprot.org