Anti-IL10 antibody (19-178) [S4793RM] (STJ11104793)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal IL10 (19-178) antibody for use in WB in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4793RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000 |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Il10 |
| Gene ID | 25325 |
| Uniprot ID | IL10_RAT |
| Immunogen Region | 19-178 |
| Immunogen Sequence | SKGHSIRGDNNCTHFPVSQT HMLRELRAAFSQVKTFFQKK DQLDNILLTDSLLQDFKGYL GCQALSEMIKFYLVEVMPQA ENHGPEIKEHLNSLGEKLKT LWIQLRRCHRFLPCENKSKA VEQVKNDFNKLQDKGVYKAM NEFDIFINCIEAYVTLKMKN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 19-178 of rat IL10 (NP_036986.2). |
Additional Info
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. |
| Protein Name | Interleukin-10Il-10Cytokine Synthesis Inhibitory FactorCsif |
| Database Links | Reactome: R-RNO-6783783 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Interleukin-10 antibodyAnti-Il-10 antibodyAnti-Cytokine Synthesis Inhibitory Factor antibodyAnti-Csif antibodyAnti-Il10 antibodyAnti-Il-10 antibody |
Information sourced from Uniprot.org