Anti-IL-6R Alpha/CD126 antibody (20-357 aa) [SRM5279] (STJ11107181)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal IL-6R Alpha/CD126 (20-357 aa) antibody for use in WB, FC and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/FC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5279 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000FC:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
Target Information
| Gene Symbol | Il6ra |
| Gene ID | 16194 |
| Uniprot ID | IL6RA_MOUSE |
| Immunogen Region | 20-357 aa |
| Immunogen Sequence | LVLGSCRALEVANGTVTSLP GATVTLICPGKEAAGNVTIH WVYSGSQNREWTTTGNTLVL RDVQLSDTGDYLCSLNDHLV GTVPLLVDVPPEEPKLSCFR KNPLVNAICEWRPSSTPSPT TKAVLFAKKINTTNGKSDFQ VPCQYSQQLKSFSCQVEILE GDKVYHIVSLCVANSVGSKS SHNEAFHSLKMVQPDPPANL VVSAIPGRPRWLKVSWQHPE TWDPSYYLLQFQLRYRPVW |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 20-357 of mouse IL-6R Alpha/CD126 (NP_034689.2). |
Additional Info
| Tissue Specificity | Expressed by dendritic cells. Soluble interleukin-6 receptor subunit alpha: Detected in the cerebrospinal fluid. |
| Post Translational Modifications | A short soluble form is also released from the membrane by proteolysis. The sIL6R is formed by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding. mIL6R is cleaved by the proteases ADAM10 and ADAM17. Glycosylated. Glycosylation is dispensable for transport, signaling, and cell-surface turnover. Glycosylation at Asn-55 is a protease-regulatory exosite. Glycosylation is required for ADAM17-mediated proteolysis. |
| Function | Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells. Interleukin-6 receptor subunit alpha: Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Soluble interleukin-6 receptor subunit alpha: Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity. The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases. In complex with IL6, is required for induction of VEGF production. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. |
| Protein Name | Interleukin-6 Receptor Subunit AlphaIl-6 Receptor Subunit AlphaIl-6r Subunit AlphaIl-6r-AlphaIl-6raIl-6r 1Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit AlphaSil6r |
| Database Links | Reactome: R-MMU-1059683Reactome: -MMU-110056Reactome: -MMU-112411 |
| Cellular Localisation | Interleukin-6 Receptor Subunit Alpha: Cell MembraneSingle-Pass Type I Membrane ProteinSoluble Interleukin-6 Receptor Subunit Alpha: Secreted |
| Alternative Antibody Names | Anti-Interleukin-6 Receptor Subunit Alpha antibodyAnti-Il-6 Receptor Subunit Alpha antibodyAnti-Il-6r Subunit Alpha antibodyAnti-Il-6r-Alpha antibodyAnti-Il-6ra antibodyAnti-Il-6r 1 antibodyAnti-Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit Alpha antibodyAnti-Sil6r antibodyAnti-Il6ra antibodyAnti-Il6r antibody |
Information sourced from Uniprot.org