Anti-IL-1 Beta antibody {ABflo® 488} (STJ11108624)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationABflo® 488
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal IL-1 Beta antibody for use in FC in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | ABflo® 488 |
| Purification | Affinity purification |
| Dilution Range | FC (intra) , 5 Mu l per 10^6 cells in 100 Mu l volume |
| Formulation | PBS containing 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis) , pH 7.3. |
| Storage Instruction | Store at 4°C for up to 1 year from the date of receipt. Do not freeze. |
Target Information
| Gene Symbol | IL1B |
| Gene ID | 3553 |
| Uniprot ID | IL1B_HUMAN |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Expressed in activated monocytes/macrophages (at protein level). |
| Post Translational Modifications | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. (Microbial infection) Cleavage by S.pyogenes cysteine protease SpeB promotes its activation independently of CASP1. |
| Function | Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection. |
| Protein Name | Interleukin-1 BetaIl-1 BetaCatabolin |
| Database Links | Reactome: R-HSA-448706Reactome: R-HSA-5620971Reactome: R-HSA-5660668Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9020702Reactome: R-HSA-9660826 |
| Cellular Localisation | CytoplasmCytosolSecretedLysosomeExtracellular ExosomeThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsSuch As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 InflammasomeCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Antibody Names | Anti-Interleukin-1 Beta antibodyAnti-Il-1 Beta antibodyAnti-Catabolin antibodyAnti-IL1B antibodyAnti-IL1F2 antibody |
Information sourced from Uniprot.org