• Immunofluorescence analysis of MCF-7 cells using IKZF3 antibody (STJ29426). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using IKZF3 Antibody (STJ29426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IKZF3 antibody (280-450) (STJ29426)

SKU:
STJ29426

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IKZF3 (280-450) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IKZF3
Gene ID: 22806
Uniprot ID: IKZF3_HUMAN
Immunogen Region: 280-450
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 280-450 of human IKZF3 (NP_036613.2).
Immunogen Sequence: HCFDVNYNSSYMYEKESELI QTRMMDQAINNAISYLGAEA LRPLVQTPPAPTSEMVPVIS SMYPIALTRAEMSNGAPQEL EKKSIHLPEKSVPSERGLSP NNSGHDSTDTDSNHEERQNH IYQQNHMVLSRARNGMPLLK EVPRSYELLKPPPICPRDSV KVINKEGEVMD
Tissue Specificity Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
Post Translational Modifications Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.
Function Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
Protein Name Zinc Finger Protein Aiolos
Ikaros Family Zinc Finger Protein 3
Cellular Localisation Nucleus
Cytoplasm
Isoform 1: Nucleus
Isoform 3: Nucleus
Isoform 11: Nucleus
Isoform 14: Nucleus
Isoform 12: Cytoplasm
Alternative Antibody Names Anti-Zinc Finger Protein Aiolos antibody
Anti-Ikaros Family Zinc Finger Protein 3 antibody
Anti-IKZF3 antibody
Anti-ZNFN1A3 antibody

Information sourced from Uniprot.org

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