Anti-IKZF1/IKAROS antibody (Internal) (STJ71205)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-IKZF1/IKAROS (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 65kDa band observed in lysates of cell line MOLT4 (calculated MW of 57.5kDa according to NP_006051.1). Recommended concentration: 0.01-0.03µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | IKZF1 |
| Gene ID | 10320 |
| Uniprot ID | IKZF1_HUMAN |
| Accession Number | NP_006051.1; NP_001207694.1; NP_001207695.1; NP_001207696.1; NP_001207697.1; NP_001207698.1; NP_001207699.1; NP_001207700.1 and more |
| Immunogen Region | Internal |
| Immunogen Sequence | DLLRAASENSQDALR |
| Specificity | This antibody is expected to recognise all reported human isoforms. |
Additional Info
| Post Translational Modifications | Phosphorylation controls cell-cycle progression from late G(1) stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G(1) phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues. Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation. Sumoylated. Simultaneous sumoylation on the 2 sites results in a loss of both HDAC-dependent and HDAC-independent repression. Has no effect on pericentromeric heterochromatin location. Desumoylated by SENP1. Polyubiquitinated. |
| Function | Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs). Function is isoform-specific and is modulated by dominant-negative inactive isoforms. |
| Protein Name | Dna-Binding Protein IkarosIkaros Family Zinc Finger Protein 1Lymphoid Transcription Factor Lyf-1 |
| Database Links | Reactome: R-HSA-9013508 Q13422-1 |
| Cellular Localisation | NucleusIn Resting LymphocytesDistributed Diffusely Throughout The NucleusLocalizes To Pericentromeric Heterochromatin In Proliferating CellsThis Localization Requires Dna Binding Which Is Regulated By Phosphorylation / Dephosphorylation EventsIsoform Ik2: NucleusIsoform Ik6: Cytoplasm |
| Alternative Antibody Names | Anti-Dna-Binding Protein Ikaros antibodyAnti-Ikaros Family Zinc Finger Protein 1 antibodyAnti-Lymphoid Transcription Factor Lyf-1 antibodyAnti-IKZF1 antibodyAnti-IK1 antibodyAnti-IKAROS antibodyAnti-LYF1 antibodyAnti-ZNFN1A1 antibody |
Information sourced from Uniprot.org