• CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g Ikaros rabbit monoclonal antibody (STJ11101900) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of Ikaros in representative gene loci (MICA) , as shown in figure.
  • Western blot analysis of extracts of various cell lines, using Ikaros rabbit monoclonal antibody (STJ11101900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunofluorescence analysis of Jurkat cells using Ikaros rabbit monoclonal antibody (STJ11101900) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros antibody (STJ11101900) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-IKZF1 antibody (250-350) [S0MR] (STJ11101900)

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STJ11101900

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Ikaros (250-350) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
ChIP 1:20-1:200
CUT&Tag 10⁵ cells/1 Mu g
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IKZF1
Gene ID: 10320
Uniprot ID: IKZF1_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Ikaros (Q13422).
Immunogen Sequence: AEDLCKIGSERSLVLDRLAS NVAKRKSSMPQKFLGDKGLS DTPYDSSASYEKENEMMKSH VMDQAINNAINYLGAESLRP LVQTPPGGSEVVPVISPMYQ L
Tissue Specificity Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.
Post Translational Modifications Phosphorylation controls cell-cycle progression from late G(1) stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G(1) phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues. Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation. Sumoylated. Simultaneous sumoylation on the 2 sites results in a loss of both HDAC-dependent and HDAC-independent repression. Has no effect on pericentromeric heterochromatin location. Desumoylated by SENP1. Polyubiquitinated.
Function Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs). Function is isoform-specific and is modulated by dominant-negative inactive isoforms.
Protein Name Dna-Binding Protein Ikaros
Ikaros Family Zinc Finger Protein 1
Lymphoid Transcription Factor Lyf-1
Database Links Reactome: R-HSA-9013508 Q13422-1
Cellular Localisation Nucleus
In Resting Lymphocytes
Distributed Diffusely Throughout The Nucleus
Localizes To Pericentromeric Heterochromatin In Proliferating Cells
This Localization Requires Dna Binding Which Is Regulated By Phosphorylation / Dephosphorylation Events
Isoform Ik2: Nucleus
Isoform Ik6: Cytoplasm
Alternative Antibody Names Anti-Dna-Binding Protein Ikaros antibody
Anti-Ikaros Family Zinc Finger Protein 1 antibody
Anti-Lymphoid Transcription Factor Lyf-1 antibody
Anti-IKZF1 antibody
Anti-IK1 antibody
Anti-IKAROS antibody
Anti-LYF1 antibody
Anti-ZNFN1A1 antibody

Information sourced from Uniprot.org

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