• Immunohistochemistry analysis of paraffin-embedded rat stomach using IKBKAP Rabbit polyclonal antibody (STJ112166) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using IKBKAP Rabbit polyclonal antibody (STJ112166) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using IKBKAP Rabbit polyclonal antibody (STJ112166) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using IKBKAP antibody (STJ112166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IKBKAP antibody (1093-1332) (STJ112166)

SKU:
STJ112166

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IKBKAP (1093-1332) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELP1
Gene ID: 8518
Uniprot ID: ELP1_HUMAN
Immunogen Region: 1093-1332
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1093-1332 of human IKBKAP (NP_003631.2).
Immunogen Sequence: LRLVYKYNRLDIIETNVKPS ILEAQKNYMAFLDSQTATFS RHKKRLLVVRELKEQAQQAG LDDEVPHGQESDLFSETSSV VSGSEMSGKYSHSNSRISAR SSKNRRKAERKKHSLKEGSP LEDLALLEALSEVVQNTENL KDEVYHILKVLFLFEFDEQG RELQKAFEDTLQLMERSLPE IWTLTYQQNSATPVLGPNST ANSIMASYQQQKTSVPVLDA ELFIPPKINRRTQWKLSLL
Function Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. ELP1 binds to tRNA, mediating interaction of the elongator complex with tRNA. May act as a scaffold protein that assembles active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
Protein Name Elongator Complex Protein 1
Elp1
Ikappab Kinase Complex-Associated Protein
Ikk Complex-Associated Protein
P150
Database Links Reactome: R-HSA-3214847
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Elongator Complex Protein 1 antibody
Anti-Elp1 antibody
Anti-Ikappab Kinase Complex-Associated Protein antibody
Anti-Ikk Complex-Associated Protein antibody
Anti-P150 antibody
Anti-ELP1 antibody
Anti-IKAP antibody
Anti-IKBKAP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance