• Confocal imaging of NIH/3T3 cells using Cation-independent M6PR (IGF2R) Rabbit monoclonal antibody (STJ11101948, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Western blot analysis of extracts of Mouse heart, using Cation-independent Cation-independent M6PR (IGF2R) (IGF2R) Rabbit monoclonal antibody (STJ11101948) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of extracts of A-549 cells, using Cation-independent Cation-independent M6PR (IGF2R) (IGF2R) Rabbit monoclonal antibody (STJ11101948) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IGF2R antibody (2392-2491) [S8MR] (STJ11101948)

SKU:
STJ11101948

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-M6PR (2392-2491) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGF2R
Gene ID: 3482
Uniprot ID: MPRI_HUMAN
Immunogen Region: 2392-2491
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2392-2491 of human Cation-independent M6PR (IGF2R) (NP_000867.3).
Immunogen Sequence: TTKSVKALSSLHGDDQDSED EVLTIPEVKVHSGRGAGAES SHPVRNAQSNALQEREDDRV GLVRGEKARKGKSSSAQQKT VSSTKLVSFHDDSDEDLLHI
Post Translational Modifications Palmitoylated. Undergoes cysteine S-palmitoylation which promotes interaction with the retromer cargo-selective complex which mediates its retrograde trafficking to the Golgi apparatus.
Function Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation by binding DPP4.
Protein Name Cation-Independent Mannose-6-Phosphate Receptor
Ci Man-6-P Receptor
Ci-Mpr
M6pr
300 Kda Mannose 6-Phosphate Receptor
Mpr 300
Insulin-Like Growth Factor 2 Receptor
Insulin-Like Growth Factor Ii Receptor
Igf-Ii Receptor
M6p/Igf2 Receptor
M6p/Igf2r
Cd Antigen Cd222
Database Links Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-6811440
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Mainly Localized In The Golgi At Steady State And Not Detectable In Lysosome
Colocalized With Dpp4 In Internalized Cytoplasmic Vesicles Adjacent To The Cell Surface
Alternative Antibody Names Anti-Cation-Independent Mannose-6-Phosphate Receptor antibody
Anti-Ci Man-6-P Receptor antibody
Anti-Ci-Mpr antibody
Anti-M6pr antibody
Anti-300 Kda Mannose 6-Phosphate Receptor antibody
Anti-Mpr 300 antibody
Anti-Insulin-Like Growth Factor 2 Receptor antibody
Anti-Insulin-Like Growth Factor Ii Receptor antibody
Anti-Igf-Ii Receptor antibody
Anti-M6p/Igf2 Receptor antibody
Anti-M6p/Igf2r antibody
Anti-Cd Antigen Cd222 antibody
Anti-IGF2R antibody
Anti-MPRI antibody

Information sourced from Uniprot.org

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