• STJ72367 (0. 5µg/ml) staining of Human Uterus (A) and negative control HepG2 (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
  • STJ72367 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72367 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-IGF1 antibody (Internal) (STJ72367)

SKU:
STJ72367-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-IGF1 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-Recommended concentration 0.5-2µg/ml
IHC-3.75µg/ml
IF-Strong expression of the protein seen in the cytoplasm of HEK293 cells. 10µg/ml
FC-Flow cytometric analysis of A549 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:3200
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: IGF1
Gene ID: 3479
Uniprot ID: IGF1_HUMAN
Immunogen Region: Internal
Accession Number: NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1
Specificity: This antibody is expected to recognize all reported isoforms (NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1).
Immunogen Sequence: RSVRAQRHTD
Protein Name Insulin-Like Growth Factor I
Igf-I
Mechano Growth Factor
Mgf
Somatomedin-C
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-2404192
Reactome: R-HSA-2428928
Reactome: R-HSA-2428933
Reactome: R-HSA-381426
Reactome: R-HSA-422085
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin-Like Growth Factor I antibody
Anti-Igf-I antibody
Anti-Mechano Growth Factor antibody
Anti-Mgf antibody
Anti-Somatomedin-C antibody
Anti-IGF1 antibody
Anti-IBP1 antibody

Information sourced from Uniprot.org

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