Anti-IFT74 antibody (1-372) (STJ114546)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IFT74 (1-372) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:3000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IFT74 |
| Gene ID | 80173 |
| Uniprot ID | IFT74_HUMAN |
| Immunogen Region | 1-372 |
| Immunogen Sequence | MASNHKSSAARPVSRGGVGL TGRPPSGIRPLSGNIRVATA MPPGTARPGSRGCPIGTGGV LSSQIKVAHRPVTQQGLTGM KTGTKGPQRQILDKSYYLGL LRSKISELTTEVNKLQKGIE MYNQENSVYLSYEKRAETLA VEIKELQGQLADYNMLVDKL NTNTEMEEVMNDYNMLKAQN DRETQSLDVIFTERQAKEKQ IRSVEEEIEQEKQATDDIIK NMSFENQVKYLEMKTTNEK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human IFT74 (NP_001092694.1). |
Additional Info
| Tissue Specificity | Highly expressed in adult and fetal kidney and expressed at lower level in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus and spleen. Detected in sperm (at protein level). |
| Function | Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis. Essential for flagellogenesis during spermatogenesis. |
| Protein Name | Intraflagellar Transport Protein 74 HomologCapillary Morphogenesis Gene 1 ProteinCmg-1Coiled-Coil Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-5620924 |
| Cellular Localisation | Cell ProjectionCiliumCytoplasmic VesicleFlagellumSecretory VesicleAcrosomeLocalizes Along Primary Cilia At Interphase And Around The Basal Body/Centriole At Interphase And MitosisIn Male Germ CellsStrongly Expressed In The Vesicles Of Spermatocytes And Round Spermatids And Also In The Acrosome And Centrosome Regions Of Elongating Spermatids And In Developing Sperm Tails |
| Alternative Antibody Names | Anti-Intraflagellar Transport Protein 74 Homolog antibodyAnti-Capillary Morphogenesis Gene 1 Protein antibodyAnti-Cmg-1 antibodyAnti-Coiled-Coil Domain-Containing Protein 2 antibodyAnti-IFT74 antibodyAnti-CCDC2 antibodyAnti-CMG1 antibody |
Information sourced from Uniprot.org