Anti-IFT20 antibody (62-112 aa) (STJ197683)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human IFT20 at the amino acid range 62-112
General Information
| Short Description | Rabbit polyclonal anti-Intraflagellar transport protein 20 homolog (62-112 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IFT20 |
| Gene ID | 90410 |
| Uniprot ID | IFT20_HUMAN |
| Immunogen | Synthesized peptide derived from the human IFT20 at the amino acid range 62-112 |
| Immunogen Region | 62-112 aa |
| Specificity | This antibody detects endogenous levels of human IFT20 |
Additional Info
| Function | Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Regulates the platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling. Essential for male fertility. Plays an important role in spermatogenesis, particularly spermiogenesis, when germ cells form flagella. May play a role in the transport of flagellar proteins ODF2 and SPAG16 to build sperm flagella and in the removal of redundant sperm cytoplasm. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment. |
| Protein Name | Intraflagellar Transport Protein 20 HomologHift20 |
| Database Links | Reactome: R-HSA-5620924 |
| Cellular Localisation | Golgi ApparatusCis-Golgi NetworkCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCilium Basal BodyCell ProjectionCiliumCytoplasmic VesicleSecretory VesicleAcrosomePresent At The Centrosomes During The Cell Cycle And Associated With The Proximal Portion Of The Mother Centriole And The Lateral Aspect Of The Daughter CentrioleAssociated With Basal Body At The Base Of Primary CiliaDetected In The Golgi Apparatus Of Round Spermatids And Late SpermatocytesAlso Detected In The Manchette Of Step 10-12 SpermatidsIn Step 14 SpermatidsFound In The Basal Body Of The Sperm TailLocalization In The Manchette Of Elongating Spermatids Is Dependent On Spag17 |
| Alternative Antibody Names | Anti-Intraflagellar Transport Protein 20 Homolog antibodyAnti-Hift20 antibodyAnti-IFT20 antibody |
Information sourced from Uniprot.org