• Western blot analysis of extracts from wild type (WT) and IFNGR1 knockout (KO) 293T cells, using IFNGR1 antibody (STJ11101380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of various lysates, using IFNGR1 antibody (STJ11101380) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IFNGR1 antibody (270-489) [S0MR] (STJ11101380)

SKU:
STJ11101380

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IFNGR1 (270-489) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFNGR1
Gene ID: 3459
Uniprot ID: INGR1_HUMAN
Immunogen Region: 270-489
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-489 of human IFNGR1 (NP_000407.1).
Immunogen Sequence: IKKINPLKEKSIILPKSLIS VVRSATLETKPESKYVSLIT SYQPFSLEKEVVCEEPLSPA TVPGMHTEDNPGKVEHTEEL SSITEVVTTEENIPDVVPGS HLTPIERESSSPLSSNQSEP GSIALNSYHSRNCSESDHSR NGFDTDSSCLESHSSLSDSE FPPNNKGEIKTEGQELITVI KAPTSFGYDKPHVLVDLLVD DSGKESLIGYRPTEDSKEFS
Post Translational Modifications Phosphorylated at Ser/Thr residues. Phosphorylation of Tyr-457 is required for IFNG receptor signal transduction. Influenza virus infection leads to phosphorylation in a CSNK1A1-dependent manner. Ubiquitinated after phosphorylation in a CSNK1A1-dependent manner, leading to the lysosome-dependent degradation. Proteasomally degraded through 'Lys-48'-mediated ubiquitination. Ubiquitination is necessary for efficient IFNGR1 signaling.
Function Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Associates with transmembrane accessory factor IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription. STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1.
Protein Name Interferon Gamma Receptor 1
Ifn-Gamma Receptor 1
Ifn-Gamma-R1
Cdw119
Interferon Gamma Receptor Alpha-Chain
Ifn-Gamma-R-Alpha
Cd Antigen Cd119
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-877312
Reactome: R-HSA-9679191
Reactome: R-HSA-9732724
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Interferon Gamma Receptor 1 antibody
Anti-Ifn-Gamma Receptor 1 antibody
Anti-Ifn-Gamma-R1 antibody
Anti-Cdw119 antibody
Anti-Interferon Gamma Receptor Alpha-Chain antibody
Anti-Ifn-Gamma-R-Alpha antibody
Anti-Cd Antigen Cd119 antibody
Anti-IFNGR1 antibody

Information sourced from Uniprot.org

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