• Immunofluorescence analysis of NIH-3T3 cells using IFNAR1 Rabbit polyclonal antibody (STJ11100540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using IFNAR1 Rabbit polyclonal antibody (STJ11100540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using IFNAR1 Rabbit polyclonal antibody (STJ11100540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using IFNAR1 Rabbit polyclonal antibody (STJ11100540) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using IFNAR1 Rabbit polyclonal antibody (STJ11100540) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using IFNAR1 Rabbit polyclonal antibody (STJ11100540) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IFNAR1 antibody (470-557) (STJ11100540)

SKU:
STJ11100540

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IFNAR1 (470-557) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFNAR1
Gene ID: 3454
Uniprot ID: INAR1_HUMAN
Immunogen Region: 470-557
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 470-557 of human IFNAR1 (NP_000620.2).
Immunogen Sequence: PSLKPSSSIDEYFSEQPLKN LLLSTSEEQIEKCFIIENIS TIATVEETNQTDEDHKKYSS QTSQDSGNYSNEDESESKTS EELQQDFV
Tissue Specificity IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
Post Translational Modifications Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex. Phosphorylated on tyrosine residues in response to interferon-binding: phosphorylation by TYK2 tyrosine kinase creates docking sites for STAT proteins. Phosphorylated on serine residues in response to interferon binding.this promotes interaction with FBXW11 and ubiquitination. Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.
Function Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response. Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another. The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors. STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes. Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway.
Protein Name Interferon Alpha/Beta Receptor 1
Ifn-R-1
Ifn-Alpha/Beta Receptor 1
Cytokine Receptor Class-Ii Member 1
Cytokine Receptor Family 2 Member 1
Crf2-1
Type I Interferon Receptor 1
Database Links Reactome: R-HSA-909733
Reactome: R-HSA-912694
Reactome: R-HSA-9679191
Reactome: R-HSA-9705671
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Late Endosome
Lysosome
Interferon Binding Triggers Internalization Of The Receptor From The Cell Membrane Into Endosomes And Then Into Lysosomes
Alternative Antibody Names Anti-Interferon Alpha/Beta Receptor 1 antibody
Anti-Ifn-R-1 antibody
Anti-Ifn-Alpha/Beta Receptor 1 antibody
Anti-Cytokine Receptor Class-Ii Member 1 antibody
Anti-Cytokine Receptor Family 2 Member 1 antibody
Anti-Crf2-1 antibody
Anti-Type I Interferon Receptor 1 antibody
Anti-IFNAR1 antibody
Anti-IFNAR antibody

Information sourced from Uniprot.org

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