Anti-IFNAR1 antibody (353-364) (STJ73000)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-IFNAR1 (353-364) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_000620.2). Recommended concentration: 0.3-1µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | IFNAR1 |
| Gene ID | 3454 |
| Uniprot ID | INAR1_HUMAN |
| Accession Number | NP_000620.2 |
| Immunogen Region | 353-364 |
| Immunogen Sequence | PKQSGNTPVIQD |
Additional Info
| Post Translational Modifications | Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex. Phosphorylated on tyrosine residues in response to interferon-binding: phosphorylation by TYK2 tyrosine kinase creates docking sites for STAT proteins. Phosphorylated on serine residues in response to interferon binding.this promotes interaction with FBXW11 and ubiquitination. Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2. |
| Function | Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response. Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another. The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors. STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes. Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway. |
| Protein Name | Interferon Alpha/Beta Receptor 1Ifn-R-1Ifn-Alpha/Beta Receptor 1Cytokine Receptor Class-Ii Member 1Cytokine Receptor Family 2 Member 1Crf2-1Type I Interferon Receptor 1 |
| Database Links | Reactome: R-HSA-909733Reactome: R-HSA-912694Reactome: R-HSA-9679191Reactome: R-HSA-9705671Reactome: R-HSA-9833109 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinLate EndosomeLysosomeInterferon Binding Triggers Internalization Of The Receptor From The Cell Membrane Into Endosomes And Then Into Lysosomes |
| Alternative Antibody Names | Anti-Interferon Alpha/Beta Receptor 1 antibodyAnti-Ifn-R-1 antibodyAnti-Ifn-Alpha/Beta Receptor 1 antibodyAnti-Cytokine Receptor Class-Ii Member 1 antibodyAnti-Cytokine Receptor Family 2 Member 1 antibodyAnti-Crf2-1 antibodyAnti-Type I Interferon Receptor 1 antibodyAnti-IFNAR1 antibodyAnti-IFNAR antibody |
Information sourced from Uniprot.org