• Immunohistochemistry analysis of paraffin-embedded human liver using IFIT3 antibody (STJ24126) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using IFIT3 antibody (STJ24126) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using IFIT3 antibody (STJ24126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-IFIT3 antibody (128-331) (STJ24126)

SKU:
STJ24126

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IFIT3 (128-331) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFIT3
Gene ID: 3437
Uniprot ID: IFIT3_HUMAN
Immunogen Region: 128-331
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 128-331 of mouse IFIT3 (NP_034631.1).
Immunogen Sequence: ANPYSMECPELECEEGWTRL KCGRNERAKMCFEKALEEKP KDPECSSGMAIAMFRLEEKP EKQFSVDALKQAMELNPQNQ YLKVLLALKLLRMGEEAEGE RLIKDALGKAPNQTDVLQKA AQFYKKKGNLDRAIELLGKA LRSTVNNSPLYSLVMCRYRE ILEQLQNKGDADSSERRQRM AELRRLTMEFMQKTLQRRRS PLNS
Tissue Specificity Expression significantly higher in peripheral blood mononuclear cells (PBMCs) and monocytes from systemic lupus erythematosus (SLE) patients than in those from healthy individuals (at protein level). Spleen, lung, leukocytes, lymph nodes, placenta, bone marrow and fetal liver.
Function IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Up-regulates CDKN1A/p21 by down-regulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.
Protein Name Interferon-Induced Protein With Tetratricopeptide Repeats 3
Ifit-3
Cig49
Isg-60
Interferon-Induced 60 Kda Protein
Ifi-60k
Interferon-Induced Protein With Tetratricopeptide Repeats 4
Ifit-4
Retinoic Acid-Induced Gene G Protein
P60
Rig-G
Database Links Reactome: R-HSA-909733
Cellular Localisation Cytoplasm
Mitochondrion
Alternative Antibody Names Anti-Interferon-Induced Protein With Tetratricopeptide Repeats 3 antibody
Anti-Ifit-3 antibody
Anti-Cig49 antibody
Anti-Isg-60 antibody
Anti-Interferon-Induced 60 Kda Protein antibody
Anti-Ifi-60k antibody
Anti-Interferon-Induced Protein With Tetratricopeptide Repeats 4 antibody
Anti-Ifit-4 antibody
Anti-Retinoic Acid-Induced Gene G Protein antibody
Anti-P60 antibody
Anti-Rig-G antibody
Anti-IFIT3 antibody
Anti-CIG-49 antibody
Anti-IFI60 antibody
Anti-IFIT4 antibody
Anti-ISG60 antibody

Information sourced from Uniprot.org

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