Anti-IFI27 antibody (1-100) (STJ116107)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IFI27 (1-100) antibody for use in WB and ELISA in rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IFI27 |
| Gene ID | 3429 |
| Uniprot ID | IFI27_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MEASALTSSAVTSVAKVVRV ASGSAVVLPLARIATVVIGG VVAVPMVLSAMGFTAAGIAS SSIAAKMMSAAAIANGGGVA SGSLVATLQSLGATGLSGLT |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IFI27 (NP_005523.3). |
Additional Info
| Post Translational Modifications | Ubiquitinated by TRIM21 via 'Lys-6'-linked ubiquitin chains leading to IFI27 mitochondrial migration. |
| Function | Probable adapter protein involved in different biological processes. Part of the signaling pathways that lead to apoptosis. Involved in type-I interferon-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9. Also functions in TNFSF10-induced apoptosis. May also have a function in the nucleus, where it may be involved in the interferon-induced negative regulation of the transcriptional activity of NR4A1, NR4A2 and NR4A3 through the enhancement of XPO1-mediated nuclear export of these nuclear receptors. May thereby play a role in the vascular response to injury. In the innate immune response, has an antiviral activity towards hepatitis C virus/HCV. May prevent the replication of the virus by recruiting both the hepatitis C virus non-structural protein 5A/NS5A and the ubiquitination machinery via SKP2, promoting the ubiquitin-mediated proteasomal degradation of NS5A. Also promotes virus-induced pyroptosis by activating CASP3 in the mitochondria after 'Lys-6'-linked ubiquitination by TRIM21. |
| Protein Name | Interferon Alpha-Inducible Protein 27 - MitochondrialP27Interferon Alpha-Induced 11.5 Kda ProteinInterferon-Stimulated Gene 12a ProteinIsg12(AIsg12a |
| Database Links | Reactome: R-HSA-909733Reactome: R-HSA-9909505 |
| Cellular Localisation | Mitochondrion MembraneMulti-Pass Membrane ProteinNucleus Inner MembraneEndoplasmic Reticulum MembraneExclusive Localizations In Either The Nucleus Or The Mitochondrion Have Been Reported |
| Alternative Antibody Names | Anti-Interferon Alpha-Inducible Protein 27 - Mitochondrial antibodyAnti-P27 antibodyAnti-Interferon Alpha-Induced 11.5 Kda Protein antibodyAnti-Interferon-Stimulated Gene 12a Protein antibodyAnti-Isg12(A antibodyAnti-Isg12a antibodyAnti-IFI27 antibody |
Information sourced from Uniprot.org