• Immunofluorescence analysis of PC-12 cells using IDS Rabbit polyclonal antibody (STJ24123) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using IDS Rabbit polyclonal antibody (STJ24123) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using IDS Rabbit polyclonal antibody (STJ24123) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using IDS Rabbit polyclonal antibody (STJ24123) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using IDS antibody (STJ24123) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IDS antibody (34-290) (STJ24123)

SKU:
STJ24123

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IDS (34-290) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IDS
Gene ID: 3423
Uniprot ID: IDS_HUMAN
Immunogen Region: 34-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-290 of human IDS (NP_000193.1).
Immunogen Sequence: TDALNVLLIIVDDLRPSLGC YGDKLVRSPNIDQLASHSLL FQNAFAQQAVCAPSRVSFLT GRRPDTTRLYDFNSYWRVHA GNFSTIPQYFKENGYVTMSV GKVFHPGISSNHTDDSPYSW SFPPYHPSSEKYENTKTCRG PDGELHANLLCPVDVLDVPE GTLPDKQSTEQAIQLLEKMK TSASPFFLAVGYHKPHIPFR YPKEFQKLYPLENITLAPDP EVPDGLPPVAYNPWMDIRQ
Tissue Specificity Liver, kidney, lung, and placenta.
Post Translational Modifications Synthesized as a 75-kDa precursor form in the endoplasmic reticulum (ER), and then processed by proteolytic cleavage through various intermediates to yield a 55-kDa mature form, with the release of an 18 kDa polypeptide. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
Function Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.
Protein Name Iduronate 2-Sulfatase
Alpha-L-Iduronate Sulfate Sulfatase
Idursulfase Cleaved Into - Iduronate 2-Sulfatase 42 Kda Chain - Iduronate 2-Sulfatase 14 Kda Chain
Database Links Reactome: R-HSA-2024096
Reactome: R-HSA-2024101
Reactome: R-HSA-2206296
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Iduronate 2-Sulfatase antibody
Anti-Alpha-L-Iduronate Sulfate Sulfatase antibody
Anti-Idursulfase Cleaved Into - Iduronate 2-Sulfatase 42 Kda Chain - Iduronate 2-Sulfatase 14 Kda Chain antibody
Anti-IDS antibody
Anti-SIDS antibody

Information sourced from Uniprot.org

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