| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow/Sheep |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-IDH1 (65-77) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
ELISA-antibody detection limit dilution 1:1000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
IDH1 |
| Gene ID: |
3417 |
| Uniprot ID: |
IDHC_HUMAN |
| Immunogen Region: |
65-77 |
| Accession Number: |
NP_005887.2 |
| Immunogen Sequence: |
KKHNVGVKCATIT |
| Function | Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency. |
| Protein Name | Isocitrate Dehydrogenase Nadp CytoplasmicIdhIdh1Cytosolic Nadp-Isocitrate DehydrogenaseIdpcNadp(+-Specific IcdhOxalosuccinate Decarboxylase |
| Database Links | Reactome: R-HSA-2978092Reactome: R-HSA-389542Reactome: R-HSA-6798695Reactome: R-HSA-9033241Reactome: R-HSA-9818025 |
| Cellular Localisation | CytoplasmCytosolPeroxisome |
| Alternative Antibody Names | Anti-Isocitrate Dehydrogenase Nadp Cytoplasmic antibodyAnti-Idh antibodyAnti-Idh1 antibodyAnti-Cytosolic Nadp-Isocitrate Dehydrogenase antibodyAnti-Idpc antibodyAnti-Nadp(+-Specific Icdh antibodyAnti-Oxalosuccinate Decarboxylase antibodyAnti-IDH1 antibodyAnti-PICD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and IDH1 knockout (KO) HeLa cells, using [KO Validated] IDH1 Rabbit polyclonal antibody (STJ119996) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98781/382830/STJ119996_1__99034.1713151333.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IDH1 knockout (KO) HeLa cells, using [KO Validated] IDH1 Rabbit polyclonal antibody (STJ119996) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")