| Short Description : | Rabbit polyclonal ID1 (1-155) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ID1 |
| Gene ID: | 3397 |
| Uniprot ID: | ID1_HUMAN |
| Immunogen Region: | 1-155 |
| Immunogen Sequence: | MKVASGSTATAAAGPSCALK AGKTASGAGEVVRCLSEQSV AISRCAGGAGARLPALLDEQ QVNVLLYDMNGCYSRLKELV PTLPQNRKVSKVEILQHVID YIRDLQLELNSESEVGTPGG RGLPVRAPLSTLNGEISALT AEAACVPADDRILCR |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of human ID1 (NP_002156.2). |
| Function | Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. |
| Protein Name | Dna-Binding Protein Inhibitor Id-1Class B Basic Helix-Loop-Helix Protein 24Bhlhb24Inhibitor Of Dna Binding 1Inhibitor Of Differentiation 1 |
| Database Links | Reactome: R-HSA-2559585Reactome: R-HSA-9031628Reactome: R-HSA-9856649 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Dna-Binding Protein Inhibitor Id-1 antibodyAnti-Class B Basic Helix-Loop-Helix Protein 24 antibodyAnti-Bhlhb24 antibodyAnti-Inhibitor Of Dna Binding 1 antibodyAnti-Inhibitor Of Differentiation 1 antibodyAnti-ID1 antibodyAnti-BHLHB24 antibodyAnti-ID antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ITCH knockout (KO) 293T cells, using [KO Validated] ITCH Rabbit polyclonal antibody (STJ118061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/96951/379801/STJ118061_1__34949.1713147667.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ITCH knockout (KO) 293T cells, using [KO Validated] ITCH Rabbit polyclonal antibody (STJ118061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
