• Immunohistochemistry analysis of paraffin-embedded rat testis using ICMT Rabbit polyclonal antibody (STJ112331) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using ICMT Rabbit polyclonal antibody (STJ112331) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ICMT antibody (STJ112331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ICMT antibody (175-284) (STJ112331)

SKU:
STJ112331

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ICMT (175-284) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ICMT
Gene ID: 23463
Uniprot ID: ICMT_HUMAN
Immunogen Region: 175-284
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 175-284 of human ICMT (NP_036537.1).
Immunogen Sequence: KAAMFTAGSNFNHVVQNEKS DTHTLVTSGVYAWFRHPSYV GWFYWSIGTQVMLCNPICGV SYALTVWRFFRDRTEEEEIS LIHFFGEEYLEYKKRVPTGL PFIKGVKVDL
Tissue Specificity Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.
Function Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
Protein Name Protein-S-Isoprenylcysteine O-Methyltransferase
Isoprenylcysteine Carboxylmethyltransferase
Prenylated Protein Carboxyl Methyltransferase
Ppmt
Prenylcysteine Carboxyl Methyltransferase
Pccmt
Database Links Reactome: R-HSA-163841
Reactome: R-HSA-9648002
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Protein-S-Isoprenylcysteine O-Methyltransferase antibody
Anti-Isoprenylcysteine Carboxylmethyltransferase antibody
Anti-Prenylated Protein Carboxyl Methyltransferase antibody
Anti-Ppmt antibody
Anti-Prenylcysteine Carboxyl Methyltransferase antibody
Anti-Pccmt antibody
Anti-ICMT antibody
Anti-PCCMT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance