Anti-IBTK antibody (900-1200) (STJ117066)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IBTK (900-1200) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IBTK |
| Gene ID | 25998 |
| Uniprot ID | IBTK_HUMAN |
| Immunogen Region | 900-1200 |
| Immunogen Sequence | GLNMAALLEARSLDVLSDGV LKDLSEFYRKMIPAMDRRVI TPYQDGPDISYLEVEDGDIF LKEEINMEQNHSETMFKKAK TKAKKKPRKRSDSSGGYNLS DIIQSPSSTGLLKSGKTNSV ESLPELLTSDSEGSYAGVGS PRDLQSPDFTTGFHSDKIEA KVKPYVNGTSPVYSREDLKP WEKSPILKISAPQPIPSNRI DTTSSASWVAGSFSPVSPPV VDLRTIMEIEESRQKCGAT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1200 of human IBTK (NP_056340.2). |
Additional Info
| Tissue Specificity | Expressed in DeFew, HEK293T, HeLa and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues. |
| Function | Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription. |
| Protein Name | Inhibitor Of Bruton Tyrosine KinaseIbtk |
| Database Links | |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinTranslocates To The Plasma Membrane Upon Igm StimulationIsoform 2: Nucleus |
| Alternative Antibody Names | Anti-Inhibitor Of Bruton Tyrosine Kinase antibodyAnti-Ibtk antibodyAnti-IBTK antibodyAnti-BTKI antibodyAnti-KIAA1417 antibody |
Information sourced from Uniprot.org